Efficient transgenesis in farm animals by lentiviral vectors
نویسندگان
چکیده
منابع مشابه
Efficient transgenesis in farm animals by lentiviral vectors.
Microinjection of DNA is now the most widespread method for generating transgenic animals, but transgenesis rates achieved this way in higher mammals are extremely low. To address this longstanding problem, we used lentiviral vectors carrying a ubiquitously active promoter (phosphoglycerate kinase, LV-PGK) to deliver transgenes to porcine embryos. Of the 46 piglets born, 32 (70%) carried the tr...
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ژورنال
عنوان ژورنال: EMBO reports
سال: 2003
ISSN: 1469-221X,1469-3178
DOI: 10.1038/sj.embor.7400007